Proteolytic activity of a rumen anaerobic fungus
نویسندگان
چکیده
منابع مشابه
In vitro study of the proteolytic activity of rumen anaerobic fungi.
Proteolytic, aminopeptidase, endopeptidase and carboxypeptidase activities of seven strains of rumen anaerobic fungi, selected to represent the fungal population commonly found in the rumen, were investigated in vitro. Whatever the nitrogen source included in the culture medium, a proteolytic activity against the 14C-labelled casein was detected in only one fungal strain. This strain belonged t...
متن کاملProteolytic activity of rumen microorganisms and effects of proteinase inhibitors.
Proteolytic activity of the bovine rumen microflora was studied with azocasein as the substrate. Approximately 25% of the proteolytic activity of rumen contents was recovered in the strained rumen fluid fraction, and the balance of the activity was associated with the particulate fraction. The proportion of proteinase activity associated with particulate material decreased when the quantity of ...
متن کاملEffects of amino acids on the growth of an anaerobic rumen fungus Neocallimastix sp N 13.
The amino acid requirements of Neocallimastix sp N 13 isolated from sheep rumen were studied using supplements containing either ten essential amino acids (10 EAA) or eight nonessential amino acids (8 NEAA), or both (18 AA). Although the fungus could grow in a medium containing ammonium sulphate as the sole source of nitrogen, amino acid supplements, especially 18 AA, greatly stimulated its gro...
متن کاملCharacterization of Heterologously Expressed Acetyl Xylan Esterase1 Isolated from the Anaerobic Rumen Fungus Neocallimastix frontalis PMA02
Acetyl xylan esterase (AXE), which hydrolyzes the ester linkages of the naturally acetylated xylan and thus known to have an important role for hemicellulose degradation, was isolated from the anaerobic rumen fungus Neocallimastix frontatlis PMA02, heterologously expressed in Escherichi coli (E.coli) and characterized. The full-length cDNA encoding NfAXE1 was 1,494 bp, of which 978 bp constitut...
متن کاملCloning, sequencing, and expression of an endoglucanase gene from the rumen anaerobic fungus Neocallimastix frontalis MCH3.
A cDNA clone encoding an endo-1,4-beta-glucanase from a rumen fungus, Neocallimastix frontalis MCH3, was isolated. The nucleotide sequence showed that the gene, celA, encoded a multidomain enzyme containing a family 5 catalytic domain and a reiterated sequence that is involved in the association of a multienzyme complex, the cellulosome. The enzyme expressed in Escherichia coli showed the highe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1985
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1985.tb00828.x